PerlPrimer - open-source PCR primer design

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The following screenshots of PerlPrimer v1.1.3 (Windows executable version) represent only some of the features of PerlPrimer. Please see the tutorial page for more screenshots of the program (running under Linux).

Standard PCR primer design with the addition of cloning sequences:

Realtime PCR primer design, showing intron-exon boundaries:

Primer information, showing Tm, thermodynamic properties and primer-dimers:

Magnified view of DNA, showing sequence, ORF translation (with codons highlighted) and the aligned primer sequence:

Primer blast search window: